Mouse serum amyloid A (SAA) elisa kit steps :
1. Dilution of standard: This kit provides one original standard, which can be diluted in small tubes according to the following chart.
release.
2. Adding samples: set blank holes separately (the blank control holes are not added with samples and enzyme labeling reagents, the other steps are the same), standard holes,
Sample hole to be tested. Accurately load 50 μl of the standard on the enzyme-labeled plate, add 40 μl of the sample dilution to the well to be tested, and then add 10 μl of the sample to be tested (the final dilution of the sample is 5 times). Add the sample to the bottom of the well of the microplate, try not to touch the wall of the well, and shake gently to mix.
3. Incubation: The plate was sealed with a sealing film and incubated at 37 ° C for 30 minutes.
4. Dosing: 30 times concentrated washing solution diluted with distilled water 30 times and used
5. Washing: Carefully remove the sealing film, discard the liquid, dry it, fill each well with the washing liquid, let stand for 30 seconds, then discard it.
Repeat 5 times and pat dry.
6. Add enzyme: 50 μl of enzyme labeling reagent was added to each well, except for blank wells.
7. Incubation: The operation is the same as 3.
8. Washing: The operation is the same as 5.
9. Color development: add 50μl of color developer A, then add 50μl of color developer B, gently shake and mix, avoid light and develop color at 37 °C
10 minutes.
10. Termination: 50 μl of stop solution was added to each well to terminate the reaction (when the blue color turned yellow).
11. Measurement: The absorbance (OD value) of each well was measured sequentially with a blank air conditioner of zero and a wavelength of 450 nm. The measurement should be carried out within 15 minutes after the addition of the stop solution. Elisa kit far from biological mouse elisa kit
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