Note on the use of eppendorf pipette during use
The eppendorf pipette, also known as the eppendorf pipette or the eppendorf pipette, is an instrument for the quantitative transfer of liquids and is widely used in the fields of biology and chemistry.
The precautions during the use of the eppendorf pipette are as follows:
1. Set the pipetting volume
Adjusting from a large range to a small range is a normal adjustment method, and rotating the scale counterclockwise;
When adjusting from small range to large range, it should be adjusted to exceed the set volume scale and then adjusted back to the set volume, which can ensure the accuracy of the pipette.
2. Assembling the pipette tip
Insert the pipette vertically into the tip, rotate it a half turn to the left and right, and tighten it.
It is highly undesirable to use a pipette to strike the tip. This long-term operation causes the pipette parts to loosen due to impact, which can cause the knob of the adjustment scale to become stuck.
3. Aspirate and discharge
Vertical aspiration;
The tip of the tip is immersed in the liquid surface below 3mm, and the tip of the tip is pre-washed in the liquid before aspiration;
Slow suction and slow release;
If the amount is small when draining, the tip of the tip should be the inner wall of the container.
4. The pipette that sucks the liquid should not lay flat. The liquid inside the pipe tip can easily contaminate the inside of the gun and may cause the spring of the gun to rust.
5. The pipette should adjust the scale to the maximum after each experiment, and let the spring return to the prototype to extend the life of the pipette.
6. When sucking the liquid, be sure to release the thumb slowly and smoothly. Never let it suddenly loosen, in case the solution is sucked too fast and flush into the liquid extractor to corrode the plunger and cause air leakage.
7. In order to obtain higher precision, the sample solution needs to be sucked once before the sample is taken, and then the liquid is officially pipetted. When the serum protein solution or organic solvent is sucked, a “liquid film†remains on the inner wall of the tip, causing the liquid discharge amount. It is too small and produces errors.
8. For liquids with large concentrations and viscosities, errors will occur. To compensate for the error, the amount of compensation can be determined by experiment. The amount of compensation can be set by changing the reading of the reading window with the adjustment knob.
9. The weight of the pure water taken by the analytical balance can be weighed and calculated to correct the liquid take-up. 1 mL of distilled water weighs 0.9982 g at 20 °C.
Do not rotate the button out of the range within the range of the pipette. Otherwise, the mechanism will be stuck and the pipette will be damaged.
10. When setting the range, please note that the number is clearly displayed in the display window and rotated to the required range.
11. Pipettes are strictly prohibited to absorb liquids with strong volatility and strong corrosiveness (such as concentrated acid, concentrated alkali, organic matter, etc.).
12. Do not use a pipette to blow the mixed liquid.
13. Do not use a large-scale pipette to remove a small volume of liquid, so as not to affect the accuracy. Also, if you need to remove a larger amount of liquid outside the range, use a pipette.
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