The third method of vitamin determination for pre-treatment solutions for food safety testing

/// Determination of vitamins A, D, and E in food (third method)

The GB 5009.82-2016 “ Measurement of Vitamins A, D and E in Foods ” issued by the National Health and Family Planning Commission of the People's Republic of China and the State Food and Drug Administration on December 23, 2016 was officially implemented on June 23, 2017. .

The first method of the new standard applies to the determination of vitamin A and vitamin E in food ; the second method applies to the determination of vitamin E in foods such as edible oil, nuts, beans and paprika ; the third method applies to vitamin D2 in foods and Determination of vitamin D3 ; the fourth method is applicable to the determination of vitamin D2 or vitamin D3 in formula foods .

Then we know that the food sample needs to be pre-processed before the analysis and testing, and the sample pre-processing time may take up 60% of the entire detection process, and the final result error of 50% may be derived from the pre-treatment process. So, for the new standard, how can IKA's pre-processing equipment help experimenters improve the efficiency and accuracy of pre-processing? Let us taste the IKA pre-processing solution by interpreting the new standards!

Determination of vitamin D in foods of the third method - Liquid chromatography - tandem mass spectrometry

experiment process

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Sample Preparation -> Sample Treatment -> Saponification

Extraction- > Evolution- > Sample Determination

GB 5009.82-2016 requirements for sample preparation:

1.1 Sample preparation: After the sample has been shrunk, pulverized or homogenized, it is stored in the dark.

Tube mill 100 tube mill

  • Can be equipped with multiple grinding cups for grinding of batch samples

  • Program control to ensure repeatability of different batches of samples

T25 digital disperser

  • Suitable for homogenization of different types of samples such as vegetables, fruits and meat

  • Special zigzag cutter head to solve the problem of sample winding

1.2 Saponification: Take 2g of homogeneous sample in a 50ml stoppered centrifuge tube --> add mixed internal standard solution and ascorbic acid / amylase, vortex for 1min --> add 12ml of ethanol, vortex for 30s --> add 6ml of hydrogen Potassium oxide, vortex 30s --> 80 ° C / 60 ° C constant temperature water bath in the dark for 30min (if the sample is relatively tight, take vortex 0.5min every 5-10min) --> cold water bath cooling --> containing The starch sample needs to be further added with ascorbic acid at 80 ° C for 30 min in the dark.

MS 3 digital vortex mixer

  • Timing function to ensure repeatability

  • Jog and continuous operation mode

  • Speed ​​up to 3000rpm for better dissolution and extraction

KS 4000i constant temperature shaker

  • RT~80°C

  • Timing function, no need to worry about saponification process

  • The universal clamp is suitable for holding centrifuge tubes and conical flasks of different specifications to achieve saponification of batch samples.

Tip: The darkening process of the saponification process can be realized in the yellow light area, as shown in the figure.

RC2 chiller

  • RT~-20°C

  • RC2 chiller can be used with different specifications of bath to achieve rapid cooling of batch samples

1.3 Extraction: cooled saponification solution plus 20ml of n-hexane -> vortex extraction 3min -> centrifugation -> transfer supernatant to 50ml centrifuge tube and water to centrifuge -> take the upper organic phase

1.4 Purification: Silica gel solid phase extraction column for over-elution -> nitrogen blow drying -> methanol vortex 30s -> filtered and tested

About IKA

IKA Group is the market leader in pre-laboratory, calorimetric analysis, mixed dispersion industrial technology. Magnetic stirrers, overhead stirrers, dispersion homogenizers, mixers, constant temperature shakers, grinders, rotary evaporators, Heating plates, constant temperature circulators, calorimeters, laboratory reactors, photobioreactors and other related products constitute the product line of IKA laboratory analysis, while industrial technology mainly includes mixing equipment for scale production, dispersing emulsification equipment, Kneading equipment, and a complete solution from pilot to expanded production. The group is headquartered in Staufen, southern Germany, with branches in the United States, China, India, Malaysia, Japan, Brazil, the United Kingdom, Poland and other countries.

Founded in 1910, IKA is now proud to review the history of the past 100 years.

Raw materials for animal medicine

Conventional veterinary drug raw materials are: oxytetracycline hydrochloride, doxycycline, chlortetracycline hydrochloride, lincomycin sulfate, gentamycin sulfate, tylosin tartrate, florfenicol, water-soluble amoxicillin, sulfamethoxazole, enno Floxacin lactate, ciprofloxacin hydrochloride, ciprofloxacin hydrochloride, metronidazole, ribavirin, vitamin C, astragalus polysaccharide, penicillin potassium.
The traditional Chinese medicine extracts in veterinary medicines are derived from natural Chinese herbal medicines.
It is purified, concentrated and purified in a different ratio from the original drug. It not only retains the natural state and biological activity of the original drug composition structure, but also retains the harmless and
The beneficial exogenous substance essence realizes the micro-quantification and standardization of Chinese herbal medicine raw materials, and provides a new way for the breeding industry to realize green production. The following herbal extracts for veterinary use: Anti-pathogenic microorganisms Chinese medicine: Anything that can inhibit or kill the reproduction of pathogenic microorganisms and improve the immunity of the body is called anti-pathogenic microorganisms
Chinese herbal medicine. According to the theory of traditional Chinese medicine, it can be divided into heat-clearing medicines, exopathic medicines, diarrhea and water-repelling medicines, damp-damping and diuretic medicines, phlegm-relieving and cough-relieving medicines, qi-regulating and Xiao-shi pharmacological blood medicines, and phlegm-relieving and anti-asthmatic medicines.

Raw materials for animal medicine,Nicarbazin in Feeds,Nosiheptide Veterinary,Nosiheptide Antibacterial,Human Gastric Mucin

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