Primary cell cryopreservation
2. The adherent cells need to be digested with 0.25% trypsin to digest the cells, and the cell suspension is collected into a centrifuge tube.
3. Centrifuge at 1000 rpm for 5 minutes and discard the supernatant.
4. Precipitate the culture medium containing DMSO, count, and adjust to (1-10)×10 6 /ml.
5. Dispense the suspension into a cryotube, 1 ml per tube.
6, seal the frozen tube, the seal must be strict, otherwise it will be prone to burst when resuscitation.
7. Mark the cell type with a marker and freeze the date.
8. Cool down in the following order: room temperature → 4 ° C (20 minutes) → refrigerator freezer (30 minutes) → ultra-low temperature refrigerator (-80 ° C overnight) → liquid nitrogen.
Chinese Herbal Medicine (English name: Chinese herbal medicine): Chinese medicine is mainly composed of botanical medicine (root, stem, leaf, fruit), animal medicine (viscera, skin, bone, organs, etc.) and mineral medicine. Because botanical medicine accounts for the majority of Chinese medicine, Chinese medicine is also called Chinese herbal medicine.
There are about 5,000 kinds of Chinese medicines used in various parts of China, and there are countless prescriptions made by combining various medicinal materials. After thousands of years of research, an independent science, Materia Medica, has been formed. Various medical schools in China have set up the course of natural medicine, which is called the Chinese herbal medicine.
The concept of plant extracts can be accepted and recognized by various countries, and it is also a common expression used to spread herbal medicines in various countries. The export value of plant extracts in China exceeded the export value of Chinese patent medicines as early as 1999. In European and American countries, plant extracts and their products (plant medicines or food supplements) have a broad market prospect, and have developed into an emerging industry with annual sales of nearly 8 billion US dollars.
The plant extracts in China are generally intermediate products, which are widely used, mainly used as raw materials or auxiliary materials for medicines, health foods, tobacco, cosmetics, etc. There are also many kinds of raw material plants used for extraction, and there are more than 300 plant varieties that are extracted industrially.
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