Introduction to the operation steps of the gel imaging system

Many manufacturers currently gel imaging system, gel imaging systems such as common on the market: imported brands roughly UVP, Wealtec, horses, Aplegen, ProteinSimple (formerly AlphaInnotech), GE, Fuji, Kodak, etc., which we have professional Jiu Ba The staff is at your service. Let's introduce the steps of gel imaging.

The steps of the gel imaging system :

1. Turn on the gel imaging system switch.

2. Turn on the computer, turn it on, and enter the imaging software.

ECL shooting

Switch the shooting mode to “ECL mode” and turn the filter wheel to the ECL position. Choose the appropriate shooting resolution (both machines with pixel merging function have this feature), click "Start". Place the sample in the middle of the sample stage, adjust the focal length of the lens so that the sample occupies about 80% of the window, then click "Auto Exposure", tick off the "negative film" and adjust the focus to make the sample image in the preview window clear. (The aperture Large, the shorter the time required for automatic exposure.) And take a single frame to shoot a photo of Mark. After the reflected white light is turned off, the nitrocellulose membrane placed on the chemiluminescent image plate is uniformly added with the luminescent liquid. Set the shooting mode to “Regular Integration”, check “Negative”, set the time and number of sheets, and click the “Shooting” button.

Ordinary gel shooting

1. Switch the shooting mode to “Normal Mode” and turn the filter wheel to the UV position (no adjustment is required without the green lens wheel).

2. Select the appropriate shooting resolution (the machine has a pixel merging function) and click "Start".

3. DNA gel shooting: Place the DNA gel in the middle of the UV station, adjust the focal length so that the sample occupies about 80% of the window, then click “Auto Exposure” and adjust the focus to make the sample image in the preview window clear. Then turn off the reflection. White light, turn on the transmission of UV, and fine-tune to ensure clear in the UV.

Protein gel shooting: Place the protein gel in the middle of the unfolded white plate, turn off the reflected white light, turn on the transmitted white light, then click “Auto Exposure” and adjust the focus to make the sample image in the preview window clear.

4. Click the “shoot” button in the software interface.

The above is "Introduction to the operation steps of the gel imaging system". For more information, please click: Gel Imaging System

In 2007, Shanghai Bayu () Industrial Co., Ltd. established an offline supply system for laboratory instruments, MRO industrial products, etc. In 2009, it officially launched an online procurement operation platform. As the earliest enterprise engaged in MRO industrial products and laboratory instruments and meters in Shanghai, Shanghai Bayu Industrial Co., Ltd. has strong strength, scientific management, quality assurance, complete after-sales and reputation first.

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