Recently, the Weizmann Institute of Science published a study in Cell Reports, which showed that T cells of the immune system can fight cancer cells more effectively after hypoxia.
A study by the Weizmann Institute of Science found that T cells after hypoxia culture are more effective in fighting cancer. Compared with mice treated with normal T cells, hypoxic T cells treated mice lived longer and their tumor shrinkage was more pronounced. However, hypoxic T cells do not penetrate tumors better than normal T cells. They are more effective against cancer because they contain higher levels of destructive enzymes, granzyme B, which can penetrate and kill. Dead cancer cells.
CAR-T cells currently used in the treatment of cancer on the market have a good effect on some cancers, such as leukemias and lymphomas, but they are not able to fight solid tumors. It is probably because the microenvironmental oxygen concentration of such tumors is extremely low: 0.5% to 5% of the oxygen is dissolved in the extracellular fluid - not only lower than most healthy organs, but also much lower than in a conventional laboratory incubator (20% oxygen can be dissolved in cell culture).
More and more researchers believe that typical cell culture conditions are hyperoxic for most types of cells, starting with more oxygen concentrations in hypoxia and nearer physiological conditions for in vitro testing. Germany BMG CLARIOstar ACU multi-function microplate reader is the world's first multi-function microplate reader to quickly restore physiological state by controlling the concentration of oxygen (O 2 ) and carbon dioxide (CO 2 ) inside the instrument. The gas environment is set completely according to the needs of the experiment. It is possible to achieve a physiological or pathological environment that is closer to the body in the most possible environment in an in vitro environment.
BMG LABTECH has just launched a new gas ramp function that enables fully automated control of the gas environment on a multi-function microplate reader, automatically achieving in vitro hypoxia, ischemia and reperfusion. This technology was first used in the gas control module (ACU) of the CLARIOstar ® multi-function microplate reader. CLARIOstar ® became the world's first to quickly restore the oxygen (O 2 ) and carbon dioxide (CO 2 ) concentrations inside the control instrument. The multi-function microplate reader in physiological state can set the gas environment completely according to the needs of the experiment in the living cell test. It is possible to achieve a physiological or pathological environment that is closer to the body in the most possible environment in an in vitro environment.
Hypoxia and ischemia-reperfusion are often associated with fatal diseases such as stroke, myocardial infarction, shock, atherosclerosis, and cancer, which can cause blood supply to be blocked resulting in a decrease in oxygen (O 2 ) and nutrient supply. The study of these changes in vitro requires a rapid and automatic control of the gas environment to automate the establishment of the pathological environment (hypoxia and automatic recovery/reperfusion), while simultaneously detecting other biological indicators in real time.
The CLARIOstar® multi-function microplate reader with gas control module (ACU) not only provides stable O 2 and CO 2 partial pressure, but more importantly, it can achieve automatic oxygen deprivation and reoxygenation. ACU's new technology enables O 2 and CO 2 partial pressure independent automatic ramp control. For example, CLARIOstar ® ACU can control hypoxia and then rapidly reoxygenate to maintain normal physiological environment while maintaining CO 2 partial pressure stability. This powerful function of constructing a disease model such as hypoxia reperfusion or tumor hypoxia in a multi-function microplate reader has greatly assisted in vitro study of living cells.
CLARIOstar ® is one of BMG LABTECH's most powerful multi-function microplate readers and is the only multi-function microplate reader on the market equipped with BMG LABTECH's revolutionary LFV grating technology. Compared to traditional grating microplate readers, CLARIOstar ® offers unparalleled sensitivity and flexibility. At the same time, temperature control, multiple oscillation mode selection, bottom reading and Z-axis focusing make CLARIOstar ® an ideal tool for long-term cell analysis.
The Gas Slope Automated Control feature is part of the MetaCellTM program and is now available and available. The MetaCellTM program team is comprised of three well-known EU high-tech companies (BMG, a German manufacturer of professional microplate readers, Luxcel biosciences, a professional R&D company for cellular energy metabolism detection reagents, and Axiogenesis AG, an internationally recognized supplier of iPSC-derived cell detection systems). It is composed of two top international research units (Oxford University and Imperial College London). The goal of the program is to establish a gold standard test platform for cell metabolism for the development of preclinical drugs.
Want to know more about the BMG multi-function microplate reader, enter the BMG microplate reader related keywords (without input serial number) in the official public number (Micro-signal: Bio-Gene): 1, cell metabolism; 2, calcium Flow; 3, prion; 4, HTS; 5, FRET and BRET; 6, BMG; 7, BMG operation
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