Open vaccination of shiitake mushrooms is a new technology that has been innovated in recent years. This technology is easy to operate, does not need inoculation box, saves time and labor, is generally welcomed by the majority of mushroom farmers.
Vaccination site selection
1. Choose a sheltered room that is sheltered from the wind, clean, dry, and well sealed. There is no source of contaminated bacteria within 50 meters.
2. The inoculation room must be cleaned and disinfected one day before the sticks are moved in.
Mushroom treatment
1. Normal pressure sterilization, after maintaining at 100 ° C for 14 hours, move the rod without mechanical damage to the inoculation chamber and cover with a film to prevent dust and spores from falling.
2. The bacteria sticks are stacked in the middle of the inoculation room, and the stack height should not exceed 1.2 meters.
3. After the bacteria rod is cooled to below 25 °C, it can be inoculated after disinfection with aerosol disinfectant for 2 hours.
Vaccination program
1. Disinfect the strains, inoculation tools, etc. with 75% alcohol.
2. Rub the inoculation site with a disinfectant or carbendazim-prepared agent.
3. Punch holes in the rubbing area, 3-4 holes per rod, the hole depth is 4-5 cm, can not be penetrated.
4. Insert the production seed into a hole, and the inoculation block and the mushroom rod should be tightly combined, but not squeezed to prevent the intrusion of bacteria from the gap.
5. When the temperature rises to 25 °C, uncover the film for ventilation, ventilation, and turn over in time to prevent high temperature burning.
Precautions
1. Inoculation should be carried out in a cold, sunny, dry weather. When the temperature is higher than 20 °C, it is rainy and foggy, and it is inoculated when windy, which has a certain influence on the survival rate.
2. The inoculation site must be clean, clean and hygienic.
3. The hands of the bacteria personnel and the punching machine must be disinfected with 75% alcohol.
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