Strengthen fertilizer and water management in terms of fertilizer needs, and nitrogen fertilizer should be the mainstay. In the early stage of growth, it mainly consumes the nutrients stored in the previous year. In order to ensure the continuity of nutrient supply, fertilizer should be applied as soon as possible at this time to supplement sufficient nutrients. In addition, the spring is dry, less rainy and windy, so water early to prepare for pomegranate flowering and fruit setting to increase the fruit setting rate.
Do a good job of cleaning up the orchard. Thoroughly remove the dead fruits, diseased and pest branches, dry and dead branches in the orchard, as well as the litter and fallen leaves scattered in the orchard, and burn them together to keep the orchard clean, clean and free of weeds.
Pruning pomegranate trees in spring. Pruning is carried out during the bud period from after germination to before flowering, usually from late April to late May. Main pruning tasks: First, adjust the ratio between fruiting branches and vegetative branches; second, thinning diseased and pest branches; third, thinning out dense shoots and main stems, and the sprouting and sprouting branches above and below the main branches to facilitate Save nutrition, while maintaining good ventilation and light permeability.
Remove the old cracked skin with a steel brush to remove the old cracked skin on the main pole, because there are many diseases and insects in the old cracked skin, it must be handled carefully.
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The RNA purification kit is used to purify and recover RNA molecules transcribed in vitro and total RNA extracted from various materials, which can effectively remove contaminating impurities in RNA samples. The recovery rate of this product can reach 80%, and the OD260/OD280 ratio of the obtained RNA is generally about 2.0, which can be directly used in subsequent sensitive experiments (such as microarray analysis, fluorescence RT-PCR, etc.). With the deepening of transcriptomics, the complexity of RNA types, expression regulation and functions is far beyond our imagination.
Removing ribosomal RNAs that account for more than 80% helps to focus sequencing on less abundant but informative RNAs. At present, the removal of rRNA is mainly through the combined use of probe and RNase H. The processed RNA will be mixed with many digestion products, enzymes and ions, which is not conducive to the subsequent construction of RNA library. Take the Columnar RNA Purification Kit as an example. Trizol is a ready-to-use reagent that can be used to purify total RNA from tissues and cells. This is a single-phase solution of phenol and guanidine isothiocyanate that facilitates lysis of tissues and cells, inhibiting RNases to maintain RNA integrity.
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