BIOG virus DNA extraction kit instructions

BIOG virus DNA extraction kit instructions (medium amount)
Transportation conditions : normal temperature transportation
Item No.: 51019-M: 10 reactions 51020-M: 20 reaction storage conditions: room temperature (15-25 ° C) , digestive juice , DA NA Carrier should be stored at -20 °C
Features
â—Ž Easy and fast operation, the ideal viral DNA can be obtained within half an hour;
â—ŽThe extracted DNA has high purity and no inhibitor, and the A260/A280 is 1.7-1.9;
â—ŽHigher output, 20% higher than domestic similar products;
â—Ž can be used for the extraction of viral DNA in body fluids such as serum, plasma, pleural and ascites, cerebrospinal fluid, and synovial fluid;
â—Ž It is free of toxic solvents such as phenol and chloroform, safe and non-toxic.
Kit composition

Component	10 times	20 times
Adsorption column and collection tube	10 each	20 each
Lysate	17mL	34 mL
Washing liquid A	31.5mL	63 mL
Washing liquid B	13.5 mL	27 mL
Eluent	10mL	20 mL
Digestive juice	1.7 mL	3.4 mL
DNA Carrier	0.35mL	0.7 mL
Instruction manual	1 copy	1 copy

product description BIOG Virus DNA Kit is a kit developed by Changzhou Baidai Biotechnology Co., Ltd. for extracting viral DNA such as serum, plasma, pleural and ascites, cerebrospinal fluid and synovial fluid. The BIOG Virus DNA Kit uses the latest high-quality imported ion membranes, and the lysate and eluent are optimized several times to efficiently separate traces of viral DNA. The extracted virus DNA has higher yield and higher purity than other similar kits of other brands, and maximizes the removal of impurities such as proteins, pigments, lipids, etc., and can be directly applied to PCR, real-time PCR and various enzyme digestion tests. Wait.
Steps

• Please prepare yourself: absolute ethanol, 15mL centrifuge tube.
• Take out the washing solution and do the following:

a) Washing solution A: 31.5 mL was added to 13.5 mL of absolute ethanol; 63 mL was added to 27 mL of absolute ethanol.
b) Washing liquid B: 13.5 mL was added to 31.5 mL of absolute ethanol; 27 mL was added to 63 mL of absolute ethanol.
c) If the prepared washing solution is precipitated, it can be dissolved at 37 ° C, shaken and used.

• Take a 15 mL centrifuge tube, add 1500 μL of sample, mix well with 30 μL of DNA Carrier, add 1500 μL of lysate and 150 μL of digestive juice, mix by shaking, and bath at 56 ° C for 10 minutes.
• Add 6000 μL of absolute ethanol and gently mix by inversion. If there is a semi-transparent suspension, it will not affect the DNA extraction and subsequent experiments.
• Put the adsorption column into the collection tube, transfer 4590 μL of the above solution into the adsorption column, let stand for 2 minutes, centrifuge at 5,000 rpm for 2 minutes, and discard the waste liquid in the collection tube;
• The adsorption column was placed back into the collection tube, and the remaining 4590 μL of the solution was transferred to the adsorption column, and step 5 was repeated.
• Put the adsorption column back into the collection tube, add 3750 μL of washing solution A to the adsorption column, centrifuge at 5,000 rpm for 1 minute, and discard the waste liquid in the collection tube.
• Put the adsorption column back into the collection tube, add 3750 μL of Washing Solution B to the adsorption column, centrifuge at 5,000 rpm for 1 minute, and discard the waste liquid in the collection tube.
• Place the column back into the collection tube and centrifuge at 5,000 rpm for 2 minutes to remove any remaining wash solution.
• The adsorption column was taken out, placed in a new 15 mL centrifuge tube, 250-400 μL of the eluate was added, allowed to stand for 3 minutes, centrifuged at 5,000 rpm for 3 minutes, and the DNA solution was collected. The extracted DNA can be used in the next experiment or at -20 °C. If you want to concentrate the nucleic acid, you can proceed to the next step.
• 800 ul of absolute ethanol was added to the eluate, gently mixed by inversion, centrifuged at 5,000 rpm for 5 minutes, and the supernatant was discarded.
• The lid was opened at room temperature for 5 min, and after the ethanol was evaporated, 30-50 ul of the eluate was added to dissolve the nucleic acid. The extracted DNA can be used in the next experiment or at -20 °C.

Recommended sample size and reagent kit component ratio:

Sample size	DNA Carrier	Digestive juice	Lysate	Absolute ethanol	Washing liquid A	Washing liquid B
1ml	20ul	100ul	1000ul	4000ul	2500ul	2500ul
1.5ml	30ul	150ul	1500ul	6000ul	3750ul	3750ul

Precautions:
The lysate and washing solution contain irritating chemicals. Please take protective measures during operation to avoid direct contact with the skin and prevent inhalation of nose and mouth. If you accidentally get into skin or eyes, rinse immediately with water or saline. Seek medical attention if necessary.

Storage, transportation and expiration date <br> This kit can be transported at room temperature, stored at room temperature (15-25 °C), valid for 12 months. Store digestive juice and DNA carrier at -20 °C to avoid repeated freezing and thawing.

Quality Control <br> This product has passed strict quality inspection and no biological pollution.
Note : This product is for scientific research only.