Automatic biochemical analyzer cross-contamination treatment advice

With the continuous development of science and technology, the medical biochemical test is fully stepped into the modernization process, and the automatic biochemical analysis instrument provides fast, correct and reproducible data for our work. However, cross-contamination of automatic biochemical analyzer is an important issue in biochemical testing and an important link leading to error. Although the production technology of biochemical analyzers is becoming more advanced, the situation of cross-contamination between reagents should also be considered. Only by considering all aspects of factors and taking effective measures, we can ensure the correctness of the inspection items and provide reliable medical equipment inspection data for the clinic. The following is a detailed introduction to the cross-contamination treatment opinions:

1. The automatic biochemical analyzer host should maintain a good working condition, which is the condition to ensure the normal operation of the work.

The OLYMPUS automatic biochemical analyzer has three probes, one for the sample needle and two for the reagent sample needle. The R1 sample needle and the R2 sample needle are the key to the correctness of the result. Therefore, the probe needle is kept clean and the inner and outer walls are smooth. It is not allowed to be soaked with strong acid when cleaning the probe. Do not use the needle to repeatedly slide the probe wall to damage the smoothness of the probe, so as to avoid increasing the cross-contamination reagent during loading. Cross-contamination of reagent needles; cross-contamination of cuvettes, probability of cross-contamination between tests, and effects when flushing probes.

2. Doing a good job in laboratory quality control and inter-room quality assessment is an indispensable means of testing the quality of our work.

We do indoor quality control day by day. If we are all within the error of error, can we explain that the report result is correct? Indoor quality control only reflects the existence of accidental error in the test, it does not mean that there is no systematic error. Hidden dangers. Cross-contamination is one of the systematic errors. Therefore, it is very important to do a good job in the inter-room quality assessment. Every time the results of the quality assessment are returned, a comprehensive summary analysis should be done to find out where the problem lies. First, whether the calibrator we selected is the best; second, we should consider whether the biochemical analyzer pipeline is contaminated, including specimen cross-contamination, reagent needle cross-contamination, cuvette cross-contamination, and reagents. Cross-contamination; the third is the exploration of test methodology. It is very important to find out the problems in our daily work, to learn to analyze the problems, and to grasp the solution to the problems, which is the basis for doing a good job.

3. Do a good job in the investigation and evaluation of cross-contamination between reagents in the experiment. There are three methods:

(1) After the measurement project you carried out, use three tubes of distilled water as the specimen to determine the item you want to evaluate. Repeat the test three times for each tube and calculate whether the results are statistically significant.

(2) Using the calibration solution as the specimen, because the cost of the calibration solution is relatively high, we only use the results obtained by repeating three times in one tube. The three results are compared. Each result is compared with the calibration value to make cross-contamination. in conclusion.

(3) Using self-made mixed serum as a specimen, the mixed serum should be used as a reagent without cross-contamination test. The method is as follows: single-input, continuous-measurement evaluation item, and 10 samples of mixed specimens are used to find a uniform value. Then do the cross-contamination test, the method is basically the same as (1), the homemade mixed serum instead of distilled water. When the results are analyzed, the method (1) and the method (2) are simultaneously carried out, so that the results of the statistical processing are comprehensive, systematic, and multi-faceted to determine whether there is cross-contamination, and the ultimate conclusion is reached.

According to the above-mentioned reagent cross-contamination investigation and evaluation, the experimental measurement procedure can be set here, and the statistically unambiguous reagents of the evaluation items are combined into adjacent reagents according to the cross-contamination investigation results between the reagents. The fair and scientific combination of reagents is the most effective way to avoid cross-contamination of reagents with an automatic biochemical analyzer. It is to ensure that the automatic biochemical analysis is more correct and provides more reliable experimental data for the clinic.